caspase-3 (1:500; ab13847; Abcam); and then incubated with secondary antibody (1:5,000; ab205718; Abcam) for. 1 h at room temperature. Protein bands were

2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681

The following day, after being washed with PBS buffer, the sections were incubated first with second-ary antibodies for 1 hour at room temperature and then with Then anti-VEGF antibody (1:200, Abcam), anti-NeuN (1:200, Millipore), anti-GFAP (1:2,000, Millipore) primary antibody were applied to the sections and incubated at 4°C overnight. .. Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature. 2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 speciﬁc antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA).

ZERO BIAS - scores, article reviews, protocol conditions and more Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation. Anti-Calreticulin Abcam ab22683 IF Anti-HMGB1 Abcam ab18256 IF Anti-LC3 Cell Signaling 2775s WB, IF Anti-ATG5 Abcam ab228668 WB Anti-ATG7 Abcam ab53255 WB Anti-p62 Abcam ab56416 WB Anti-caspase3a Abcam ab2302 WB Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB detection method by using a mouse and rabbit specific Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3 antibody, anti-active Caspase 3 antibody active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 body (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4°C overnight. The following day, after being washed with PBS buffer, the sections were incubated first with second-ary antibodies for 1 hour at room temperature and then with Then anti-VEGF antibody (1:200, Abcam), anti-NeuN (1:200, Millipore), anti-GFAP (1:2,000, Millipore) primary antibody were applied to the sections and incubated at 4°C overnight. .. Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature. 2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 speciﬁc antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting.

I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information.

2020-12-01 · Briefly, the specimen was fixed in 10% formaldehyde and embedded in paraffin. The slices went through deparaffinage, rehydration, antigen retrieval, endogenous peroxidase blockage, and nonspecific signal blocking. Next, the slices were incubated with an anti-Caspase3 antibody (Abcam, ab13847, 1:200) at 4 °C overnight.

ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team

I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information. Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Abcam caspase-3 antibody (Abcam, ab13847) was used in immunocytochemistry on human samples at 1:100 (fig s9) and in western blot on human samples at 1:1000 (fig s9). Nat Commun (2016) ncbi domestic rabbit polyclonal active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR-21, and nonspecific Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB domestic rabbit monoclonal (E61) reactivity: human conjugate: HRP application: western blot, immunohistochemistry - paraffin section The sections were incubated with primary antibodies anti-MVH (ab13840, Abcam), LC3B (Ab51520, Abcam), CASP9 (Ab202068, Abcam), CASP3 (Ab13847, Abcam), and Stat3 (9139p, Cell signaling technology Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation. Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active).

Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands.
Preflight parking phoenix

Company Abcam; Price Pricing Info Supplier Page View Company Product Page; Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Knockout Tested Rabbit recombinant monoclonal Caspase-3 antibody [EPR18297].

A8K5M2, D3DP53, P42574, Q96AN1 ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.
Pension plan specialists

fittja vårdcentral boka tid
reg nr transport
paradigmatic meaning
money exchange göteborg
avspenning kryssord
pengar drivkraft
traditionellt hantverk

active Caspase 3 human, reacts with human, mouse, rat, fruit fly (drosophila melanogaster), indian muntjac, rhesus monkey, xenopus laevis and zebrafish (pmid 19823566)predicted to react with chinese hamster (100 identity with immunogen), dog (100 identity with immunogen) and pig (100 identity with immunogen) due to sequence homology

Knockout Tested Rabbit recombinant monoclonal VCAM1 antibody [EPR5047]. Validated in WB, IP, ELISA, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 136 publication(s). Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Abcam catalog: ab13847 total number of products: 19. domestic rabbit polyclonal reactivity: human, mouse, rat, Xenopus laevis application: western blot 产品报价：询价更新时间：2013/6/20 13:23:01 产地：美国.